Single molecule spectroscopic/microscopic method development
and applications to biophysics with an emphasis on the role of dynamics in
enzyme functions. Methods of interest include single molecule fluorescence
resonance energy transfer, precise localization of macromolecules by
fluorescence and light scattering, single photon correlation spectroscopy, and
optical trapping. Systems of interest include molecules and assemblies involved
in gene transcription and translation.
Single molecule biophysics
Single molecule spectroscopy/microscopy provides ways to
monitor sub-population dynamics, thereby yielding valuable information
complementing ensemble measurements. High sensitivity imaging devices and
several innovative signal processing techniques have boosted popularity of
single molecule methods in many fields of science and engineering. A few recent
additions of single molecule methods to the field of biophysics include single
molecule fluorescence resonance energy transfer, nanometer (co)localization of
single fluorophores, rotational and translational motion tracking of single
particles, and single molecule manipulation with optical or magnetic tweezers.
Studying dynamics of molecules is essential in understanding their
behavior and function in cells. Studying dynamics of asynchronously moving
molecules through a multi-step enzymatic reaction requires capability of
monitoring sub-population dynamics, which can be efficiently achieved with
various single molecule methods. My research program focuses on the dynamics of
molecules involved in several steps of gene transcription and translation in
order to understand what roles dynamics of molecules play in the processes.
Various single molecule imaging techniques and optical tweezers are utilized to
probe and control the dynamics of the molecules involved in the proposed
systems. My research program is very much interdisciplinary across physical
chemistry, physics, biochemistry, molecular/structural/cellular biology, and
nanoscale science/engineering. The goal is to provide i) novel insights into
the link between the mechanisms of complex cellular machineries and the
fundamental laws of dynamics, and ii) efficient single molecule methods to
study and control complex enzymatic reactions.
Biophysics post-doc position openings
I am currently looking for post-doctoral researchers. Individuals trained in
biological sciences or experimental physical sciences are strongly encouraged to
apply. Enthusiastic scientists trained in other disciplines searching for career
opportunities in single molecule biophysics are also welcome to apply. Potential
roles in the lab include routine work in cellular/molecular biology, application
and/or development of spectroscopic/microscopic techniques to the above
If interested, please send your CV, including references, to email@example.com.